Frozen Semen Characteristics of Limousin Bull at Different Ages

Strategies to increase the population and productivity of beef cattle can be implemented through reproductive management, such as artificial insemination (AI) using frozen semen. This study aims evaluate characteristics semen Limousin bulls different ages, which still used for programs up 12 years age. ages 3, 8, produced in 2021 at Artificial Insemination Center Singosari, East Java Province, Indonesia. Five from each age group were replication. Computer-assisted sperm analysis was determine motility, viability, abnormalities eosin–nigrosine staining. Plasma membrane integrity analyzed hypoosmotic swelling test. The acrosomes’ evaluated FITC-PNA-PI, protamine deficiency chromomycin A3, DNA fragmentation testing acridine orange fluorescent technique. results showed that parameters abnormality, plasma integrity, no significant differences all groups. kinematic (straightness beat cross frequency) 3-year-old significantly higher (p<0.05) compared with those other Parameters acrosome a prevalence Furthermore, 12-year-old research concludes increasing reduce fragmentation.